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. 2001 May 29;98(12):6656–6661. doi: 10.1073/pnas.131067798

Figure 1.

Figure 1

Colocalization of the α- and β-subunits of ATP synthase on the surface of HUVECs by immunostaining and confocal microscopy. (a) Nonpermeabilized HUVECs immunostained with a murine mAb specific for the α-subunit of ATP synthase. (b) The same cells immunostained with a rabbit polyclonal antiserum specific for the β-subunit of ATP synthase. (c) Composite colocalization images obtained by digital overlays of the above images. (d) Colocalization image obtained from cells permeabilized with ethanol (100%). Representative images are shown; n = 26.