Figure 3.

Binding of angiostatin to purified bovine F₁ ATP synthase. ELISA was used to determine concentration-dependent binding of angiostatin to a constant amount of F₁ ATP synthase. Each well was coated with 1 μg of F₁ ATP synthase before addition of decreasing amounts of angiostatin. Control lane (−) shows binding of secondary antibody only (n = 6). (Inset) Apparent dissociation constants [K_d(app)] were determined from double-reciprocal plots of the binding data. Angiostatin bound to bovine F₁ ATP synthase with a K_d(app) of 14.1 nM.