Figure 3.  .

Immunodetection of lacritin in horse tears. (A) Right (OD) and left (OS) eye tear samples from three horses, a human tear sample (Hu Tears), and recombinant human lacritin [Lac(recomb)] were separated by SDS-PAGE and then Coomassie blue stained. Many proteins are apparent in horse tears, some in molecular weight regions not commonly seen in human tears. (B) Horse and human tear samples and recombinant lacritin were separated by SDS-PAGE and blotted. Blots were incubated with anti-C-terminal lacritin antibodies using peroxidase-conjugated secondary antibody. Bound antibodies were detected by chemiluminescence. Lacritin detected in horse tears is smaller than that in human tears and may be a C-terminal fragment. (C) Blotted horse and human tear samples and recombinant lacritin were incubated with anti-N-terminal lacritin antibodies using peroxidase-conjugated secondary antibody and chemiluminescent detection. A band corresponding in size to human tear lacritin was very weakly detected.