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. 2012 Aug 26;2012:945131. doi: 10.1155/2012/945131

Figure 2.

Figure 2

Nitric oxide and cytokine production in peritoneal macrophages collected from mice fed with control diet (CD) or high-fat diet (HFD), supplemented or not with sunflower oil (rich in n-6 fatty acids, 2 g/Kg b.w., three times a week, by oral gavage). Animals were fed with HFD or CD for eight weeks. Peritoneal macrophages were collected and cultured for 24 h in the absence (white bars) or presence (black bars) of 2.5 μg/mL LPS. Nitric oxide (a), TNF-α (b), IL-6 (c), and IL-10 (d) were measured as described in Material and Methods. Total number of cells in peritoneal exudates was not different among the groups (data not shown). Data are presented as mean ± SEM (n = 5–10). The CD + n6 group had just 5 samples up to the detection limit in the TNF-α assay. Two-way ANOVA and Bonferroni post-test were used for statistical analysis. All LPS stimulated conditions were different from unstimulated; *P < 0.05 versus CD with LPS; #P < 0.05 versus CD + n6 with LPS; °P < 0.05 versus HFD with LPS.