Figure 4.

Resolution of IR-induced nuclear foci. (A) PC12 cells expressing AR10Q or AR112Q were cultured with R1881 and stained with antibodies against γ-H2AX or 53BP1 before (zero) or 1, 4 or 24 h post irradiation. (B) The percentages of cells with 10 or more γ-H2AX-positive nuclear foci were counted in eight independent images for each time point. (C) The percentages of cells with 10 or more 53BP1 nuclear foci were counted from eight fields at each time point. Statistically differences were calculated by the Student's t-test; *P< 0.01 for all comparisons except the 1-h γ-H2AX samples.