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HU and SFN do not inhibit class I and class II histone deacetylase (HDAC) activities. HDAC activity was measured by an in vitro colorimetric assay using whole cell extracts from HeLa cells or normal human fibroblasts. A decrease in A405 correlates with a reduction in HDAC activity. *P ≤ 0.00009; paired t-test. Bars = SEM for n ≥ 3 independent experiments performed in triplicate.
