Figure 3.

PRPF39 knockdown significantly increases the resistance of four cell lines to cisplatin. After successful PRPF39 knockdown using siRNA, cisplatin cytotoxicity was assessed using the alamarBlue growth inhibition assay. For four different LCLs, IC₅₀ values after successful knockdown of PRPF39 are significantly higher relative to a non-targeting control (19100 P= 0.025; 19252 P= 0.017; 18925 P= 0.069; 18488 P= 0.067), demonstrating that after knockdown cell lines are more resistant to cisplatin. P-values are from a Student's one-tailed t-test based on a minimum of three separate knockdown experiments. When incorporating the four different cell lines into a single mixed effects model, the P-value drops to 0.0001, with the IC₅₀ of the knockdown being ∼34% above the non-targeted control.