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. Author manuscript; available in PMC: 2012 Sep 13.
Published in final edited form as: Mod Pathol. 2011 Apr 15;24(8):1120–1127. doi: 10.1038/modpathol.2011.56

Figure 1.

Figure 1

Schematic representation of ERG rearrangement identification by tricolor FISH. The red probe hybridizes to the 3′ sequence of ERG, the green probe hybridizes to the 5′ sequence of ERG, and the aqua probe hybridizes to TMPRSS2. (a) Wild-type (normal) findings at the 21q22 locus show a triplet of overlapping red and green signals with aqua signals spaced at a variable distance from the red–green pair. (b) Cells with ERG–TMPRSS2 rearrangement exhibit splitting (separation) of the red–green signal pair, accompanied by a fused red–aqua signal for one allele. (c) A subset of cases with rearrangement showed loss of the corresponding green signal for one allele, indicating 5′ ERG deletion.