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. 2012 Aug 22;3:299. doi: 10.3389/fmicb.2012.00299

Figure 1.

Figure 1

Comparison of CA-immunosuppression and neutrophil depletion and their effects on IA survival. (A) Kaplan–Meier survival plots of CF-1 mice i.n. infected with three million viable A. fumigatus conidia. Curves show CA-immunosuppressed mice with non-specific rat IgG as controls (Rat IgG/CA, triangles, n = 9), CA-immunosuppressed mice with anti-GR1 antibody (anti-GR1/CA, open triangles, n = 10), neutrophil-depleted mice (anti-GR1, circles, n = 12), and non-immunosuppressed mice with non-specific rat IgG (Rat IgG, squares, n = 12). (B,C) FACS analysis of Ly6C+ GR1+ neutrophils in tail blood from control mice (left panels, Rat IgG) and neutrophil-depleted mice (right panels, anti-GR1; (B)] without CA-immunosuppression and (C) with CA-immunosuppression. The circle marks the population of neutrophils coated with the unlabelled anti-GR1 antibody. (D) Binding of anti-rat antibody to anti-GR1 IgG-coated neutrophils in CA-immunosuppressed mice from the circled population in (C) (right panel, anti-GR1) compared to the isotype control (left panel, Rat IgG). (E) Effect of anti-GR1 antibody on the depletion of monocytes, CD8+ T cells and neutrophils in mouse blood, n = 3; *statistically significant, p < 0.05.