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. 2001 Jun 5;98(12):6680–6685. doi: 10.1073/pnas.111163898

Figure 2.

Figure 2

IFNγ regulates gene and protein expression in Stat1−/− macrophages. Fifteen μg of total RNA from macrophages treated with either buffer or IFNγ (14 ng/ml) for different periods of time were subjected to Northern analyses by using probes specific for MIP-1α (A), CXCR4 (C), IRF-1, Arginase, IL-1β (E), and β-actin. The quantitation of mRNA expression was performed by using a Molecular Dynamics PhosphorImager with the corresponding IMAGEQUANT software. (B) Twenty million BMM derived from WT (■) or Stat1−/− (♦) mice were incubated with or without IFNγ (14 ng/ml). After the indicated times the supernatant was harvested, concentrated 7-fold by ultrafiltration and screened by ELISA for MIP-1α (Quantikine M from R&D Systems). The result shown is representative of three experiments. (D) One million BMM were incubated with or without IFNγ (14 ng/ml) for 10 or 24 h. The analysis of calcium mobilization, after the addition of SDF-1α (0.01 mM), was performed as described in Materials and Methods.