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. 2012 May 20;2:27. doi: 10.1186/2191-0855-2-27

Figure 1.

Figure 1

Schematic plasmid map of pKOV410-mCherry-YlCWP1expression vector and of the cell wall fusion proteins. ( A) mCherry-YlCWP1 fusion gene in plasmid pKOV410- mCherry-YlCWP1 under the transcriptional control of hp4d promoter, pre-pro Lip2 for secretion of fusion proteins and terminator (Term). The plasmid contains URA3d4 and KanR markers for selection. ( B) mCherry-YlCWP1 covalently binds to β-1,6-glucan in the cell wall of Y. lipolytica through glycosylphosphatidylinositol (GPI) anchor. The plasmid map was constructed using Geneious v5.5 ([Drummond et al. 2011]).