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Fluorescent immunohistochemical images and maps. Tumors were triple-stained with fluorescent immunohistochemical markers of hypoxia (pimonidazole), endothelium (9 F1), and perfusion (Hoechst 33342), to produce images of hypoxia ( A), endothelium ( B), perfusion ( C), and a combined image ( D). Corresponding maps were extracted and used in the quantitative analysis ( E-G). Composite map where artefacts and necrosis, which were excluded from the measurements, are shown in white ( H).
