Figure 2.

Validation and clustering analysis of developmentally regulated miRNAs.A-C. The expression of three groups of miRNAs at different developmental stages revealed by deep-sequencing. The three groups are: gradual increasing (A), gradual decreasing (B), and peak at middle stages (C). The expression level of miRNAs (TPM) at each developmental stage was normalized to that at E10. D-F. Quantitative PCR (qPCR) analysis of the expression profile of different groups of miRNAs shown in A-C. Results are based on average of three independent experiments (Mean ± SD). The developmental changes of the expression of most miRNAs revealed by qPCR are consistent with results from deep-sequencing. G. Clustering of differentially expressed microRNAs. The Complete Linkage Clustering was used by R package based on expression levels (TPM) of each miRNA at different stages. Both known miRNAs and novel miRNAs are included (Dataset S1). Red means highly expressed and green means lowly expressed.