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. 2012 Jun 19;10(1):7. doi: 10.1186/1897-4287-10-7

Table 2.

Sequence variants identified in theBRCA2gene

Nucleotide Change AA change Number of patients Clinical Relevance (BIC) Variation class§ Polyphen SIFT Align GVGD
Frameshift mutations
5804_5808delTTAA
I1859KfsX3
1
Deleterious
5
 
 
 
c.9485-1 G > A*
 
1
Not reported
Not reported
 
 
 
Missense variations
353A > G
Y42C
1
Unknown
1
+++
Tolerated
Class 0
1093A > C
N289H
6
No effect
1
+
Not tolerated
Class 0
1342A > C
N372H
20
No effect
1
+
Tolerated
Class 0
3199A > G
N991D
2
Unknown
1
++
Tolerated
Class 0
5972 C > T
T1915M
2
Unknown
1
++
Tolerated
Class 0
6359 G > C
G2044A
1
Unknown
3
+
Tolerated
Class 0
6550 C > T
R2108C
1
Unknown
3
+++
Tolerated
Class 0
7625 C > T
A2466V
26
Unknown
1
+
Not tolerated
Class 0
9079 G > A
A2951T
1
No effect
2
+
Not tolerated
Class 55
Nonsense mutations
10204A > T
K3326X
1
No effect
1
 
 
 
Synonyms
1593A > G
S455S
6
No effect
1
 
 
 
2457 T > C
H743H
6
Unknown
1
 
 
 
3624A > G
K1132K
16
No effect
1
 
 
 
4035 T > C
V1269V
13
No effect
1
 
 
 
4791A > G
L1521L
38
No effect
2
 
 
 
5718 C > T
S1830S
1
Not reported
Not reported
 
 
 
6741 C > G
V2171V
38
Unknown
1
 
 
 
7470A > G
S2414S
13
No effect
1
 
 
 
10338 G > A
R3370R
1
Unknown
1
 
 
 
UTRs and Intronic variations
203 G > A
5’UTR
16
No effect
Not reported
 
 
 
IVS4 + 67A > C
 
6
Unknown
1
 
 
 
IVS4 + 147 G > T
 
4
Not reported
Not reported
 
 
 
IVS6 + 14 C > T
 
1
Unknown
3
 
 
 
IVS7 + 183 T > A
 
21
Not reported
1
 
 
 
IVS7-9A > G
 
1
Unknown
Not reported
 
 
 
IVS8 + 56 C > T
 
8
Unknown
1
 
 
 
IVS10-34 C > A
 
1
Not reported
3
 
 
 
IVS10-51 G > T
 
1
Not reported
1
 
 
 
IVS11 + 80delTTAA
 
10
Unknown
3
 
 
 
IVS12-120 T > C
 
37
Not reported
3
 
 
 
IVS13 + 133insATTATAAAA
 
5
Not reported
Not reported
 
 
 
IVS13 + 273 G > A
 
2
Not reported
Not reported
 
 
 
IVS14 + 53 C > T
 
4
No effect
Not reported
 
 
 
IVS16-14 T > C
 
25
No effect
1
 
 
 
IVS21-66 T > C
 
25
No effect
1
 
 
 
IVS22 + 53 C > G
 
1
Not reported
Not reported
 
 
 
IVS24-16 T > C
 
1
Unknown
1
 
 
 
10485 + 105A > C 3’UTR 15 Not reported Not reported      

*Missense considered deleterious.

Polyphen: +++ Variant predicted to be probably damaging, ++ Variant predicted to be possibly damaging, + Variant predicted to be benign.

UMD-BRCA1/2: 1 Variant considered neutral, 2 Variant considered likely-neutral, 3 Variant considered unclassified, 4 Variant considered likely-causal, 5 Variant considered causal.

§ in the UMD-BRCA1 database (http://www.umd.be/BRCA1/) (12) using classification system in five classes (13).