Figure 3.

Effect of cold chain storage on gametocyte mRNA preserved on filter papers. Percentage of mRNA amplification in 10, 1.0 and 0.1 gametocyte/μl dilutions, determined by QT-NASBA (open bars) and RT-PCR (hatched bars), when stored on (a) the Whatman 903 Protein Saver filter paper and (b) the Whatman FTA Classic Card, under cold chain conditions with variable humidity for up to thirteen weeks. The background blue to pink colour gradient represents the severity of the incubation condition (from cold to warm, respectively).