Table 1.
Comparison of DNA yield and quality between the MagAttract kit and this improved method
| Tissue Type | Method | Total DNA Yield (μg per sample) | OD260nm/OD280nm | OD260nm/OD230nm |
|---|---|---|---|---|
| Leaves |
MagAttract kit |
1.7 ± 0.4† |
2.16 ± 0.22 |
0.65 ± 0.09 |
| This method |
49.8 ± 9.1* |
1.88 ± 0.02 |
2.35 ± 0.03 |
|
| Seeds |
MagAttract kit |
N/A |
N/A |
N/A |
| This method | 30.5 ± 12.1* | 1.90 ± 0.02* | 2.24 ± 0.11* |
† Average yield of 14 successful extractions and standard deviation.
* Average yield of a 96-well plate and the standard deviation.