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. 2012 May 30;12:73. doi: 10.1186/1471-2229-12-73

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Copyright ©2012 Jiang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Characterization of HMW-GSs isolated fromAegilopssect.sitopsisspecies. (a) SDS-PAGE analysis revealed that both x-type and y-type HMW GSs (marked by arrow and triangle, respectively) are expressed in three Aegilops accessions and all six subunits have the molecular weights larger than those of Chinese Spring. (b) The complete DNA ORFs of both x-type and y-type HMW-GSs were amplified from S genome. Lane 1, Ae. bicornis; Lane 2, Ae. longissima; Lane 3, Ae. sharonensis; CS, Chinese Spring; M is the 1Kb DNA ladder.