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. 2012 May 30;12:73. doi: 10.1186/1471-2229-12-73

Table 1.

Comparison of the N-terminal amino acid sequences derived from protein sequencing with those deduced from the cloned DNA sequences encoding for HMW-GS in three Aegilops species

Species Subunit Residue
1 2 3 4 5 6 7 8 9 10 11 12
Triticum aestivum
 x-type consensus
 E
 G
 E
 A
 S
 G/E
 Q
 L
 Q
 C
 E
 R/H
Ae. bicornis
 1Sbx sequenced
 E
 G
 E
 A
 S
 G
 Q
 L
 Q
 C
 E
 R
1Sbx deduced
 E
 G
 E
 A
 S
 G
 Q
 L
 Q
 C
 E
 R
Ae. longissima
 1Slx sequenced
 E
 G
 E
 A
 S
 G
 Q
 L
 Q
 C
  
  
1Slx deduced
 E
 G
 E
 A
 S
 G
 Q
 L
 Q
 C
 E
 R
Ae. sharonensis
 1Sshx sequenced
 E
 G
 E
 A
 S
 G
 Q
 L
 Q
 C
 E
  
1Sshx deduced
 E
 G
 E
 A
 S
 G
 Q
 L
 Q
 C
 E
 R
T. aestivum
 y-type consensus
 E
 G
 E
 A
 S
 R/K
 Q
 L
 Q
 C
 E
 R
Ae. bicornis
 1Sby sequenced
 E
 G
 E
 A
 S
 R
 Q
 L
 Q
 C
 E
 R
1Sby deduced
 E
 G
 E
 A
 S
 R
 Q
 L
 Q
 C
 E
 R
Ae. longissima
 1Sly sequenced
 E
 G
 E
 A
 S
 R
 Q
 L
 Q
 C
 E
  
1Sly deduced
 E
 G
 E
 A
 S
 R
 Q
 L
 Q
 C
 E
 R
Ae. sharonensis
 1Sshy sequenced
 E
 G
 E
 A
 S
 R
 Q
 L
 Q
 C
 E
 R
  1Sshy deduced E G E A S R Q L Q C E R

The consensus sequences of x-type and y-type HMW subunits of bread wheat were also list as standard. The results indicated that the amino acid sequences translated from the cloned genes are perfectly matched to those of the native protein of seeds.