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. 2012 Aug 21;9:165. doi: 10.1186/1743-422X-9-165

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Copyright ©2012 Hou et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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IL-10 was up-regulated in PRRSV-infected macrophages. PAMs and BMDMs were infected with PRRSV or UV-inactivated virus at an MOI of 1. Cells stimulated with LPS (1 μg ml^-1) were used as positive control. (a), (c), (e) Cells were harvested at the indicated time points and real-time PCR was performed to evaluate IL-10 mRNA levels. (b), (d), (f) Measurement of secreted IL-10 protein levels in the supernatant of PRRSV-infected PAMs and BMDMs at indicated time points by ELISA. The results represent means ± SD of three independent experiments. *Significant difference (P < 0.05) from media control using Student’s t-test.