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PRRSV infection activated p38 MAPK signaling pathway in BMDMs. Whole-cell extracts of BMDMs infected with (a) PRRSV at an MOI of 1 or (b) equal amounts of UV-inactivated virus were prepared at indicated time points and analyzed by immunoblot for the phosphorylation status of p38 (p-p38) and ERK1/2 (p-ERK1/2). The blot was also probed for tubulin as loading control.
