Figure 5.

Intraperitoneal administration of 3PO suppresses the development of imiquimod-induced psoriasis. (A) Three BALB/c mice were administered a daily topical dose of 62.5 mg of imiquimod cream (5%) over a shaved area on the back for 5 days. Thirty minutes following each daily administration of imiquimod, the mice were injected intraperitoneally with either DMSO or 3PO 0.07 mg/gm daily. After 5 days, photographs were taken that demonstrate gross skin folds (i.e. wrinkling), scaling and erythema. A photograph was captured of a shaved mouse that had not been treated with imiquimod (Normal). (B), After 5 daily topical applications of imiquimod, skin thickness was measured using microcalipers. (C) After 5 daily topical applications of imiquimod, the epidermal thickness relative to the total cutis was determined. (D) After 5 daily topical applications of imiquimod, spleens were resected and mass was determined. Data from nine mice were averaged from three independent experiments (n = 3 per experiment). *p < 0.05 (relative to vehicle control). Data are representative of three independent experiments.