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. 2012 Aug 2;11:50. doi: 10.1186/1476-4598-11-50

Figure 3.

Figure 3

Inactivation of Flna reduces proliferation of K-RASG12D–expressing primary mouse embryonic fibroblasts (MEF). (A) Genotyping of MEF by genomic PCR using F1 and R2 primers, as indicated in Figure 1, for Flna before (left lanes) and after (right lanes) infection with adenoviral vector encoding Cre (Ad-Cre). M, DNA ladder. (B) Genotyping of MEF by genomic PCR using F4 and R4 primers, as indicated in Figure 1, for Kras2 after infection with Ad-Cre. Upper band represents insertion of a single loxP site after removal of LSL in the Kras2 gene (Kras2G12D). (C) Proliferation assay of K-RAS–induced MEF with or without Flna up to 4 days. Wild-type MEF cells were included as controls. (D) Representative immunoblots of FLNA, phosphorylated ERK (p-ERK) and AKT (p-AKT) in K-RAS–induced cells with or without Flna after infection with Ad-Cre. Actin loadings and total ERK (t-ERK) and AKT (t-AKT) immunoblots served as internal controls. Mean ± SD values of densitometric readings of band intensities in triplicate experiments. Student’s t test, *P < 0.05, **P < 0.01.