Table 2. Parameters for reconstruction.

	Image Preprocess	Fragmental line structures assembling	Reconstruct the 3D centerlines	Polygonal path approximation
Initial threshold level	10, in a 256-gray scale image	_	_	_
Threshold for the 2D 8-neighbor CC	9	_	_	_
Threshold for the 3D 26-neighbor CC	30	_	_	_
Maximum edge weight to connect two components	_	5% of the largest image dimension	_	_
Radius R	_	_	1.0 µm	_
η	_	_	10	_
Ratio for determine branches in the soma	_	_	0.2	_
Threshold for connecting to a point in BpList	_	_	1.0 µm	_
Threshold for connecting to a point in Bps2D	_	_	0.75 µm	_
ε for path approximation