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. 2012 Jun 18;7(1):309. doi: 10.1186/1556-276X-7-309

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Copyright ©2012 Ruan et al.; licensee Springer.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Morphology and immunophenotypic characterization of MSCs. (A) The fibroblastic morphology of passage 3 MSCs (magnification = ×100); (B) MSCs stained with FITC-conjugated CD29 antibody (×200); (C) MSCs stained with PE-conjugated CD90 antibody (×200); (D), (E), and (F) MSCs analyzed by FACS for the positive expression of CD29 (D) and CD90 (E) and negative expression of CD45 (F); (G) Differentiated osteoblasts tested with alkaline phosphatase staining (×100); (H) Differentiated adipocytes characterized by oil red O staining (×100); (I) Differentiated chondrocytes verified by toluidine blue staining (×100).