Figure 4. Eugenol induces Ca²⁺ influx from intracellular and extracellular Ca²⁺ sources in stationary phase cells.

Ca²⁺-dependent aequorin luminescence from JK9-3da (A), cch1Δ (B), mid1Δ (C) and cch1Δmid1Δ (D) cells in stationary growth phase in response to 0.6 mg/ml eugenol. Eugenol was added at 40 seconds and was suspended in either SCM-leu (SCM) or EGTA buffer (EGTA). EGTA* represents luminescence from JK9-3da and cch1Δ cells cultured overnight in SCM-leu supplemented with 10 mM CaCl₂. Traces represent mean (± SEM) from at least 4 independent experiments. SEM values are illustrated using grey shading. Luminescence was recorded every 0.2 seconds and is expressed in arbitrary units (AU).