Skip to main content
. 2012 Sep 13;7(9):e37835. doi: 10.1371/journal.pone.0037835

Figure 5. The effects of GJC on PKAI, GPR3 levels and cAMP surge in oocytes of COCs stimulated by FSH.

Figure 5

(A) Carbenoxolone (CBX) completely suppressed FSH-induced GVBD. COCs were preincubated for 0.5 h in HX medium containing 100 µM CBX. Then FSH was added to some COC cultures, and the cultures were maintained for 22 h. Percent GVBD was then determined. The bar marked with the asterisk is significantly different from the other bars. (B) CBX suppressed FSH-induced cumulus expansion of COC. HX, control; FSH, 50 IU/L FSH; HX+CBX, HX medium supplemented with 100 µM CBX; FSH+CBX, HX medium supplemented with 50 IU/L FSH and 100 µM CBX. Scale bar, approximately 50 µm. (C) cAMP levels in cumulus cells (CCs) of COCs preincubated with CBX and then treated with FSH. (D) cAMP levels in oocytes of COCs preincubated with CBX and then treated with FSH. A mean of 100 COCs were used at each time point in each of three replicate experiments. The mean ± SEM of three individual experiments are shown. (E) Inhibition of oocyte-cumulus cell GJC abolished FSH-induced changes in PKAI and GPR3 levels. COCs were preincubated for 0.5 h in HX medium containing 100 µM CBX. Then FSH was added to the culture medium. DOs and CCs from 200 COCs treated/not treated with FSH were collected at various time points and analyzed by Western blotting. The blot shown is representative of three experiments.