Table 2.
Percent positivity values of test serum samples
| |
Protein for immobilization* |
Neospora caninum iscomELISA kit | |||
|---|---|---|---|---|---|
| Sample number |
MBP- NcGRA2 |
MBP- NcSRS2 |
NcSAG1 | Co- immobilization | |
| Positive |
100 |
100 |
100 |
100 |
100 |
| Negative |
10.0 |
3.5 |
10.1 |
8.3 |
5.3 |
| 1 |
56.5 |
48.3 |
51.5 |
53.4 |
27.6 |
| 2 |
47.3 |
52.5 |
58.2 |
53.2 |
30.0 |
| 3 |
57.0 |
43.2 |
22.4 |
45.3 |
27.6 |
| 4 |
59.3 |
37.8 |
51.0 |
56.0 |
39.0 |
| 5 |
36.0 |
29.5 |
53.1 |
39.1 |
41.7 |
| 6 |
19.0 |
21.2 |
28.8 |
27.6 |
35.9 |
| 7 |
18.5 |
13.0 |
14.8 |
16.8 |
20.5 |
| 8 |
92.1 |
73.0 |
78.3 |
81.4 |
56.6 |
| 9 |
45.2 |
22.5 |
30.2 |
31.5 |
26.8 |
| 10 |
45.6 |
42.1 |
37.8 |
39.5 |
50.1 |
| 11 |
53.2 |
36.8 |
27.2 |
40.6 |
38.4 |
| 12 |
15.3 |
12.3 |
10.2 |
14.3 |
4.1 |
| 13 |
10.0 |
3.4 |
5.3 |
7.6 |
6.2 |
| 14 |
12.3 |
4.8 |
11.8 |
10.6 |
17.5 |
| 15 |
4.1 |
5.7 |
2.4 |
3.9 |
6.3 |
| 16 |
7.3 |
4.2 |
5.6 |
5.6 |
10.3 |
| 17 |
4.3 |
5.0 |
8.3 |
6.2 |
2.3 |
| 18 |
5.6 |
4.1 |
5.2 |
5.0 |
3.0 |
| 19 |
5.3 |
4.8 |
7.2 |
6.5 |
5.3 |
| 20 |
7.1 |
3.7 |
4.4 |
5.8 |
3.2 |
| 21 |
3.2 |
2.5 |
6.5 |
4.2 |
2.1 |
| 22 |
13.3 |
9.0 |
8.9 |
11.0 |
2.4 |
| 23 |
9.5 |
6.4 |
8.2 |
7.8 |
2.9 |
| 24 |
6.6 |
10.2 |
8.1 |
8.8 |
2.1 |
| 25 |
4.5 |
11.4 |
4.7 |
5.8 |
3.3 |
| 26 |
5.1 |
8.3 |
6.0 |
7.0 |
2.7 |
| 27 |
5.2 |
4.7 |
3.8 |
4.0 |
3.0 |
| 28 |
8.0 |
4.3 |
4.8 |
5.1 |
3.4 |
| 29 |
9.3 |
5.9 |
7.3 |
7.4 |
3.6 |
| 30 | 6.2 | 3.6 | 4.2 | 4.7 | 4.7 |
*The concentrations of MBP-NcGRA2, MBP-NcSRS2 and NcSAG1 were 1.0, 0.5, and 0.25 μg/ml for both individual and co-immobilization.