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. 2012 Sep 13;7(9):e45360. doi: 10.1371/journal.pone.0045360

Figure 4. ITSN1 and Ras form a BiFC complex.

Figure 4

(A) VC-tagged ITSN1 was co-transfected with one of the following VN-tagged Ras constructs: WT, 61L, 17N, or 17N/69N. ITSN1 formed a complex (green) with Ras17N >WT >17N69N >61L. CFP (red) was used as a transfection control. (B) The graph represents the average fluorescence intensity per cell ± S.E.M. from at least three independent experiments (*p<0.05, **p<0.01). (C) A Western blot was performed to demonstrate equal expression of all constructs. (size bar  = 20 μm).