Table 4.
Predicted Chlamydomonas reinhardtii miRNAs that are responsive to sulfur-deprivation
| Name | Sequence (5'-3') | L (nt) | Location in the geonome | MFE (kcal mol-1) | Reads +S/-S |
|---|---|---|---|---|---|
| n006 | UCCAGCUGGGCGGCCGUCUCC | 21 | chromosome_10:4733426:4733510:- | -38.6 | 1004/2798 |
| n015 | UUCUACCCAAGAGGCUGUGUA | 21 | chromosome_12:2479175:2479337:+ | -93.9 | 84/264 |
| n030 | UCAAAGCUAGGAGCCAUGAAG | 21 | chromosome_14:139326:139506:+ | -129.4 | 1347/4625 |
| n046 | UGUUCGGAGAUCCUUGUGCAUG | 22 | chromosome_16:3072001:3072107:- | -101.1 | 117/373 |
| n051 | UUGUUGACGACGUGCGCGGGC | 21 | chromosome_17:204043:204314:- | -129.8 | 41137/86593 |
| n052 | UGACACAUGGAACAACACAAC | 21 | chromosome_1:3554627:3554814:+ | -150.9 | 287/505 |
| n062 | UGACAUGCGGUGAAUGUGAAU | 21 | chromosome_3:5564702:5564806:+ | -105.4 | 15185/49088 |
| n077 | UCAUGAAGCGGAUACUGUGAA | 21 | chromosome_7:743887:744064:- | -103.91 | 1160/61 |
| n083 | UGGGCCUGUUGUGCACGUUCC | 21 | scaffold_28:208520:208678:- | -115.9 | 104/662 |
| n084 | UUGUGCCGGCCGACACUGCGG | 21 | scaffold_33:45408:45519:+ | -49.9 | 77092/55878 |
| n152 | UGUACGGCGACCUGCAAAUGG | 21 | chromosome_10:5980485:5980652:+ | -115.7 | 0/215 |
| n168 | AGGAUGACCGUCAUGAUUGCG | 21 | chromosome_13:4630466:4630659:- | -167 | 0/4628 |
| n169 | GUCAUUAAGACCGUCGGCAAU | 21 | chromosome_14:1815533:1815835:+ | -206.26 | 0/170 |
| n182 | UAGGGCUUUUCGGAAGGGAGA | 21 | chromosome_17:4302900:4303054:+ | -83.2 | 0/20463 |
| n196 | AUUCACAUUCACCGCAUGUCA | 21 | chromosome_3:5564693:5564816:+ | -112 | 0/16257 |
| n197 | UCCUCCUCCUUGACGUCGGCG | 21 | chromosome_3:7309395:7309503:+ | -50.1 | 0/321 |
| n198 | AGCAGCUUCCCACUCCCACGACC | 23 | chromosome_3:2104211:2104422:- | -66.5 | 0/415 |
| n200 | UGCGCAGCGGCAUCAUCUGGA | 21 | chromosome_4:2994696:2994894:- | -157.7 | 0/367 |
| n207 | UAGCAGUCUGAACCAAAGUCG | 21 | chromosome_7:2564607:2564734:+ | -92.1 | 0/931 |
| n209 | UAUGGGCAGUUGUACUAAAUC | 21 | chromosome_7:4354369:4354571:+ | -136.8 | 0/564 |
| n212 | UGGGCCUCACGGCGGCGGACC | 21 | chromosome_7:5449979:5450131:+ | -140.8 | 0/270 |
| n214 | AGGGCCAACAGCUUUGACCGG | 21 | chromosome_7:5670376:5670487:+ | -103.9 | 0/557 |
| n222 | AAUGCCAGCAGCUCCACGCCC | 21 | scaffold_18:257:618:+ | -158.6 | 0/4384 |
Name: the name of predicted Chlamydomonas reinhardtii miRNAs that are responsive to sulfur-deprivation; Sequence: sequence cloned in small RNA libraries; L, the length of miRNA; MFE: the adjusted minimum free energy (MFE) representing the MFE of 100 nucleotides; Reads: the effect of sulfur-deprivation on miRNA expression, +S/-S, normalized sequencing frequencies in the +S and -S library.