Figure 2. Slc15a4 is required for antigen specific T cell responses in vivo.

8 days after LCMV Cl13 infection, splenocytes from WT and feeble mice were cultured ex vivo with the indicated peptides at 10⁻⁷ M, 10⁻⁸ M (“lo” concentration) or without peptide for 5 hours and then stained with antibodies to quantitate T cell antigen specific production of IFN-γ (A) and TNF-α (B). In (C) mice were primed with TAP1−/− 5E1 fibroblasts expressing the model antigen from Adeno E1B. Seven days after i.p. injection splenocytes from WT, feeble, and inept mice were cultured ex vivo with the immunodominant antigen for 5 hours. Then IFN-γ expression was quantitated in CD8⁺ T cells by flow cytometry. Mean and standard error of the mean are shown. feeble, Slc15a4^{feeble/feeble} mice; inept, IRF7^inept/inept mice. Representative data of 2 independent experiments are shown. n≥4 per group of mice. Unless marked, p>0.05 between WT and feeble and not statistically significantly different.