Figure 4. PDGFRα overcomes gH/gL/UL128–131 mediated interference in ARPE-19 epithelial cells.
ARPE-19 epithelial cells were transduced with A) Ad-tet-trans alone, B) Ad-PDGFRα and Ad-tet-trans, C) Ad vectors expressing all of gH, gL, UL128, UL130 and UL131 and Ad-tet-trans, D) Ad- PDGFRα and vectors expressing gH, gL, UL128, UL130, UL131 and Ad-tet-trans for 24 hr. The cells were incubated with HCMV TR using 10 IU/cell for a further 24 hr, then the cells cells fixed, permeabilized, and stained for HCMV IE-86 early antigen. E) The percent of HCMV infected cells was quantified as described in Fig. 2. Error bars indicate the standard deviation.