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. 2004 Feb;24(4):1595–1607. doi: 10.1128/MCB.24.4.1595-1607.2004

FIG. 1.

FIG. 1.

PARP-2 interacts with TRF2. (A) Lysates from Cos1 cells expressing Myc-hTRF2 fusion protein (lanes 1 to 5) together with either GST (lane 1) or GST-mPARP-2 fusion protein (lanes 2 to 5) were analyzed by GST pull down under increasing stringency conditions of washing buffers as indicated, followed by Western blotting using, successively, anti-Myc (top) and anti-GST (bottom) antibodies. (B) Conditions of interaction between PARP-2 and TRF2. Interaction of GST (lane 1) or GST-mPARP-2 fusion protein (lanes 2 to 5) with Myc-hTRF2 fusion protein (lanes 1 to 5) in Cos1 cells either untreated (lane 2) or treated with 4 mM N-nitroso-N-methylurea (lane 3), 2 mM 3-AB (lane 4), or 10 μg of ethidium bromide/ml (lane 5). Proteins were analyzed by GST pull down and Western blotting using, successively, anti-Myc, anti-GST, and antipolymer antibodies as indicated.