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. 2012 Sep 13;7(9):e45240. doi: 10.1371/journal.pone.0045240

Figure 3. Quantification of lymphatic capillary development after knockdown with a vegfc morpholino.

Figure 3

Red fluorescent images from late-phase microangiograms of Tg(fli1:EGFP)y1 zebrafish are shown, highlighting Texas Red-LMD uptake within lymphatic capillaries. Results of quantitative morphometric analyses are displayed in bar graphs. A and B, Zebrafish injected with a vegfc morpholino (MO) have significant inhibition of lymphatic capillary growth at 3 dpf (n = 32) compared to untreated zebrafish (n = 32) or those exposed to rhVEGF-C (n = 14), which is mitigated by the addition of rhVEGF-C (100 pg/ml) to the aqueous environment (n = 15). *P<0.0001, **P<0.0001. Scale bars, 50 µm.