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. 2012 Sep 13;7(9):e45240. doi: 10.1371/journal.pone.0045240

Figure 5. Quantification of lymphatic capillary development after inhibition with small molecules.

Figure 5

Red fluorescent images from late-phase microangiograms of Tg(fli1:EGFP)y1 zebrafish are shown, highlighting Texas Red-LMD uptake within lymphatic capillaries. Results of quantitative morphometric analyses are displayed in bar graphs. A and B, Addition of hVEGFR-3 inhibitor in DMSO (n = 30) or rapamycin in DMSO (n = 18) to the aqueous environment significantly reduced lymphatic capillary development at 3 dpf, compared to zebrafish exposed to DMSO alone (n = 21 and 15, respectively). *P<0.0001, **P<0.0001. Scale bars, 50 µm.