Skip to main content
. 2012 Sep 13;7(9):e44984. doi: 10.1371/journal.pone.0044984

Figure 2. The JHA1 isolate is lethal to immunocompetent mice and causes tissue damage.

Figure 2

(A) Survival curves of male Balb/c mice infected i.c. with different viral loads (25 to 300 PFU) in a final volume of 40 µL. Mice were monitored for 21 days after the challenge. (B) Body weight loss in mice infected with the JHA1 strain. The body weights of infected and mock-infected animals were monitored daily, and the differences between the initial and final measurements were calculated and are presented as percentages. (C) Hematocrit determination in mice infected with the JHA1 strain. The animals were bled on the seventh day after the challenge. (D) Serum LDH levels in JHA1-infected mice. The serum samples were collected 7 days after the challenge. Statistically significant differences were determined with the ANOVA test and a subsequent Bonferroni’s multiple comparison test. Statistically significant differences are indicated with asterisks: *, p<0.05; **, p<0.01; and ***, p<0.001. Data are representative of three independent experiments.