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. 2012 Sep 13;7(9):e44984. doi: 10.1371/journal.pone.0044984

Figure 4. Determination of viable virus particles and induction of inflammatory and adaptive immune responses in mice infected with the JHA1 strain.

Figure 4

(A) Replication of the JHA1 strain in brain and spleen tissues. Mice infected i.c. with 300 PFU were euthanized on day 7 p.i., and both the brains and spleens were removed and processed as described in the Methods section. (B) Quantification of INF-γ levels in the serum samples of mice infected with the JHA1 strain. The serum samples were collected seven days after the challenge. (C) Virus-specific serum IgG responses induced in mice infected with the JHA1 strain. The serum samples collected on days 2, 4, 6 and 7 p.i. were tested individually for the presence of NS1- or EIII-specific antibodies by ELISA. Background values detected in serum samples collected from mock-treated animals were reduced from those measured among virus-infected mice.