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. 2012 Jun 24;12:8. doi: 10.1186/1471-5945-12-8

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Copyright ©2012 Marcelo et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Micrographs of ePUK cultures. 3a: A complete monolayer is shown with ePUK cells in overlying medium. 3b: Adhering keratinocytes cells attached from cells suspended in spent medium when suspension was passed into a new flask. This micrograph was a taken approx. 16 hours after suspension was poured into new flask. 3c: A new monolayer formed after 2–3 days. 3d: A monolayer, after a number of generations (PnSnpnsn) of ePUK culturing demonstrated the very small population of epithelial cells that dominate an optimum monolayer grown using ePUK technology. Bar=50 microns for a,b,c,d.