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. 2012 Sep 13;8(9):e1002910. doi: 10.1371/journal.ppat.1002910

Figure 1. dotL mutants are defective for intracellular growth.

Figure 1

(A) Schematic of the dotL mutants. Twelve dotL mutants, each containing a single amino acid change, were identified. A black line indicates their individual locations within the 783 amino acid protein DotL and the residue changed is shown. The DotL transmembrane domains are shown by a hatched box and the Walker A box motif (GSTGSGKT) is shown by a gray box. A. castellanii (B) and A/J mouse bone marrow derived macrophages (C) were infected with a wild-type strain (Lp02) (filled squares), a T4SS-deficient strain (Lp03) (filled triangles), a ΔdotL strain containing a dotL+ complementing clone (filled inverted triangles) and two representative dotL mutants (Q222R (open diamonds) and Y725Stop (open circles)). Fold growth was calculated by dividing the average colony forming units (CFUs) of triplicate wells at a given time point by the average CFUs at time zero. Error bars represent the standard deviation from the mean and the results are representative of three independent experiments.