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. 2012 Sep 13;8(9):e1002910. doi: 10.1371/journal.ppat.1002910

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© 2012 Sutherland et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Subcellular localization of IcmS was assayed using L. pneumophila lysates separated into total (T), soluble (S) and membrane (M) fractions. ΔA ΔL is the ΔdotA ΔdotL double mutant and all of the dotL mutants were expressed in a ΔdotL background. Protein fractions were analyzed by western blot using an IcmS-specific antibody. Fractions were loaded proportionally and are representative of three independent experiments.