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. 2012 Sep 13;8(9):e1002910. doi: 10.1371/journal.ppat.1002910

Figure 7. The dotLY725Stop mutant has a specific secretion defect for IcmSW-dependent substrates.

Figure 7

Export of CyaA fusions to the following Dot/Icm substrates was assayed: A. SdeA, B. SidD, C. VipA, D. RalF, E. LnaB, and F. LidA. The CyaA:substrate fusions were expressed in a wild-type strain (black bars), a strain lacking icmS (white bars), a strain with the dotLY725Stop mutant integrated onto the chromosome (striped bars), and a dotLY725Stop ΔicmS double mutant (gray bars). cAMP was measured by ELISA from triplicate wells and error bars represent the standard deviation from the mean.