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. 2004 Feb;24(4):1779–1790. doi: 10.1128/MCB.24.4.1779-1790.2004

FIG. 4.

FIG. 4.

Restoration of polyA-dependent translation by addition of exogenous PABP. (A) Luc RNA translation in HeLa extracts treated with buffer or 3Cpro and supplemented with His-PABP. Black bars represent the percentages of translation (light units relative to buffer control levels) in buffer- or 3Cpro-treated extracts. Gray-shaded bars indicate Luc translation levels after the addition of His-PABP. (B) Immunoblot of wild-type (WT) or mutant (Q537E) His-PABP left untreated or treated with 3Cpro. Cleavage fragments of PABP are indicated (3C cp and 3Calt cp). (C) Luc RNA translation in extracts incubated with wild-type (wt) or Q537E PABP. (D) Translation of polyadenylated or nonpolyadenylated RNA in buffer- or 3Cpro-treated extracts (black bars) supplemented with wild-type (wt) PABP (empty bars) or Q537E PABP (dark-gray-shaded bars). Data in panels A, C, and D represent the means ± SD of the results from three individual experiments.