FIG. 5.

PABP-eIF4GI interactions are not abolished during poliovirus infection. (A) Immunoblot analysis of eIF4G (upper panel) and PABP (lower panel) in complexes isolated from mock (M)- or poliovirus (PV)-infected HeLa lysates by the use of poly(A) agarose. (B) Immunoblot analysis of eIF4GI (upper panel) or PABP (lower panel) in complexes isolated from mock (M)- or poliovirus (PV)-infected HeLa lysates by the use of m⁷GTP-Sepharose. Cleavage fragments of PABP or eIF4GI (i.e., PABP cp_N and eIF4G cp_N) are indicated. (C) Immunoblot analysis of eIF4GI in complexes isolated from uninfected HeLa lysates by the use of His-PABP or the N-terminal fragment of His-PABP truncated at the 3Calt cleavage site (amino acid 413) by insertion of a stop codon (His-PABP₄₁₃). Recombinant proteins were expressed in BLR(DE3) bacterial cells and purified on Talon beads before being used in pulldown assays. BLR indicates bacterial lysate from nontransformed cells (used as a negative control); the control was HeLa lysate incubated alone with Talon beads.