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. 2004 Feb;24(4):1628–1639. doi: 10.1128/MCB.24.4.1628-1639.2004

FIG. 7.

FIG. 7.

Prolonged elevation of intracellular calcium upon disruption of the actin cytoskeleton. (A) Cytochalasin D (CytD) treatment prolongs Ca2+ flux in T cells stimulated with anti-CD3 MAb. Th1 T-cell clones were labeled with Ca2+ binding dyes FuraRed and Fluo3, followed by incubation with culture medium or cytochalasin D (2 μM) for 1 h at 37°C. Cells were then incubated for 1 h on ice with 5 μg of anti-CD3 MAb per ml. Cells were then warmed to 37°C, and Ca2+ flux was measured by flow cytometry after the addition of goat anti-hamster antiserum. (B) Ca2+ flux is prolonged by cytochalasin D in T cells stimulated with thapsigargin. Th1 T cells were labeled as described above and incubated with culture medium or cytochalasin D for 1 h at 37°C. Cells were then stimulated with thapsigargin, and Ca2+ flux was measured. (C) Cytochalasin D prolongs elevated levels of intracellular Ca2+ in T cells stimulated with P+I. Labeled Th1 T cells were incubated with culture medium or cytochalasin D for 1 h at 37°C. Ca2+ flux was measured following the addition of P+I.

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