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. 2012 Sep 13;8(9):e1002920. doi: 10.1371/journal.ppat.1002920

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Human polyclonal antibody off-rate constants that describe the fraction of antibody-antigen complexes decaying per second were determined directly from the plasma sample interaction with H1N1pdm09-HA1 (A) and H1N1pdm09-HA2 (B) proteins during the dissociation phase for pre- and post-vaccination samples from each individual. Fold change in plasma antibody off-rate constants (each symbol represents one individual) were determined as described in Materials and Methods. (A) The fold decrease in antibody off-rates following H1N1pdm09 vaccination strongly correlated with AID fold-change. (B) No significant correlation was observed in antibody affinity against HA2 and AID fold change. (C) Positive correlation between AID activity in B cells before vaccination (t0) and fold change in antibody affinity in t28 vs. t0. The off-rate constants were determined from two independent SPR runs.