Figure 3.

Detection of apoptosis of U373MG cells by exposure to the anti-cancer chemical compounds. U373MG cells were exposed to the chemical compounds individually at the concentration of IC₅₀ for 72 hours or 20 μM GSK3B inhibitor VII for 36 hours. They were then processed for western blot analysis. The panels (A-D) represent the blot of (A) ET-770, the compound 1a, (B) ET-770 derivative, the compound 3, (C) RM, the compound 2a, and (D) GSK3B inhibitor VII. The panels (a-l) indicate the blot of (a, d, g, j) poly-ADP-ribose-polymerase (PARP) composed of an 116-kDa uncleaved product and an 85-kDa cleaved product, (b, e, h, k) caspase-3 (CASP3) composed of 19-kDa and 17-kDa cleaved products, and (c, f, i, l) heat shock protein HSP60, an internal control of protein loading. The lanes (1–16) represent the cells (1, 7) untreated or treated for (2, 8) 4 hours, (3, 9) 8 hours, (4, 10) 24 hours, (16) 36 hours, (5, 11) 48 hours, and (6, 12, 14) 72 hours with the compounds or (13) 72 hours with the vehicle (DMSO), and (15) 36 hours with the vehicle (DMSO).