Table 2.
KEGG pathways of the genes downregulated or upregulated in U373MG cells following exposure to anti-cancer chemical compounds
|
ET-770 (the Compound 1a) |
|
ET-770 Derivative (the Compound 3) |
|
RM (the Compound 2a) |
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
|
Downregulated Genes (n = 426) |
|
Downregulated Genes (n = 417) |
|
Downregulated Genes (n =274) |
|
||||||
| Rank | Pathway | P-Value | FDR | Rank | Pathway | P-Value | FDR | Rank | Pathway | P-Value | FDR |
| 1 |
hsa04012:ErbB signaling pathway |
0.0006 |
0.71 |
1 |
hsa04510:Focal adhesion |
0.0001 |
0.12 |
1 |
hsa04070:Phosphatidylinositol signaling system |
0.0003 |
0.38 |
| 2 |
hsa04360:Axon guidance |
0.0008 |
0.94 |
2 |
hsa04360:Axon guidance |
0.0002 |
0.21 |
2 |
hsa04916:Melanogenesis |
0.0019 |
2.17 |
| 3 |
hsa04070:Phosphatidylinositol signaling system |
0.0041 |
4.69 |
3 |
hsa04810:Regulation of actin cytoskeleton |
0.0007 |
0.80 |
3 |
hsa05223:Non-small cell lung cancer |
0.0027 |
3.03 |
| 4 |
hsa04520:Adherens junction |
0.0052 |
5.82 |
4 |
hsa04020:Calcium signaling pathway |
0.0083 |
9.21 |
4 |
hsa04510:Focal adhesion |
0.0032 |
3.51 |
| 5 |
hsa05214:Glioma |
0.0076 |
8.42 |
5 |
hsa04012:ErbB signaling pathway |
0.0093 |
10.22 |
5 |
hsa04020:Calcium signaling pathway |
0.0043 |
4.69 |
| |
|
|
|
6 |
hsa04514:Cell adhesion molecules (CAMs) |
0.0097 |
10.63 |
6 |
hsa04012:ErbB signaling pathway |
0.0046 |
5.10 |
| |
|
|
|
|
|
|
|
7 |
hsa05214:Glioma |
0.0053 |
5.86 |
| |
|
|
|
|
|
|
|
8 |
hsa04720:Long-term potentiation |
0.0074 |
8.01 |
| |
|
|
|
|
|
|
|
9 |
hsa04912:GnRH signaling pathway |
0.0083 |
8.91 |
| |
|
|
|
|
|
|
|
10 |
hsa04360:Axon guidance |
0.0084 |
9.08 |
|
Upregulated Genes (n = 45) |
|
Upregulated Genes (n = 84) |
|
Upregulated Genes (n = 9) |
|
||||||
|
Rank |
Pathway |
P-Value |
FDR |
Rank |
Pathway |
P-Value |
FDR |
Rank |
Pathway |
P-Value |
|
| 1 | hsa04110:Cell cycle | 0.0053 | 4.58 | 1 | hsa04110:Cell cycle | 0.0077 | 6.84 | none | |||
The genome-wide gene expression profile was studied on Human Gene 1.0 ST array in U373MG cells following a 24 hour-exposure to the vehicle (DMSO) or to the three chemical compounds (Figure 1). By importing the complete list of Entrez Gene IDs of downregulated or upregulated genes into the Functional Annotation tool of DAVID, the KEGG pathways related to the set of imported genes showing p < 0.01 by a modified Fisher Exact test were identified. The pathways are listed with the category, p-value, and false discover rate (FDR %).