Table 3.

IPA networks of the genes downregulated or upregulated in U373MG cells following exposure to anti-cancer chemical compounds

ET-770 (the Compound 1a)			ET-770 Derivative (the Compound 3)			RM (the Compound 2a)
Downregulated Genes (n = 426)			Downregulated Genes (n = 417)			Downregulated Genes (n = 274)
Rank	Associated Network Functions	P-Value	Rank	Associated Network Functions	P-Value	Rank	Associated Network Functions	P-Value
1	Cellular Assembly and Organization, Cellular Function and Maintenance, Molecular Transport	1.00E-45	1	Cardiovascular Disease, Tissue Development, Post-Translational Modification	1.00E-43	1	Cellular Movement, Cardiac Arteriopathy, Cardiovascular Disease	1.00E-48
2	Tissue Development, Cellular Movement, Nervous System Development and Function	1.00E-36	2	Cell-To-Cell Signaling and Interaction, Cellular Assembly and Organization, Cellular Movement	1.00E-40	2	Gene Expression, Cellular Growth and Proliferation, Cell Death	1.00E-38
3	Developmental Disorder, Skeletal and Muscular Disorders, Nervous System Development and Function	1.00E-34	3	Cell-To-Cell Signaling and Interaction, Nervous System Development and Function, Cellular Function and Maintenance	1.00E-34	3	Cellular Movement, Cell Morphology, Cell-To-Cell Signaling and Interaction	1.00E-35
4	Gene Expression, Infectious Disease, Cellular Development	1.00E-34	4	Cellular Movement, Cellular Growth and Proliferation, Carbohydrate Metabolism	1.00E-31	4	Cellular Growth and Proliferation, Cellular Movement, Cell Death	1.00E-29
5	Post-Translational Modification, Cellular Assembly and Organization, Cellular Function and Maintenance	1.00E-28	5	Gene Expression, Cellular Development, Cellular Growth and Proliferation	1.00E-31	5	Cellular Movement, Nervous System Development and Function, Developmental Disorder	1.00E-28
Upregulated Genes (n = 45)			Upregulated Genes (n = 84)			Upregulated Genes (n = 9)
Rank	Associated Network Functions	P-Value	Rank	Associated Network Functions	P-Value	Rank	Associated Network Functions	P-Value
1	Cell Cycle, Gene Expression, Cell Death	1.00E-41	1	Cellular Growth and Proliferation, Cell Cycle, Cell-To-Cell Signaling and Interaction	1.00E-39	1	Cellular Development, Cellular Growth and Proliferation, Cancer	1.00E-14
2	Inflammatory Response, Lipid Metabolism, Small Molecule Biochemistry	1.00E-27	2	Gene Expression, Infectious Disease, Cell-To-Cell Signaling and Interaction	1.00E-32	2	Cell-To-Cell Signaling and Interaction, Molecular Transport, Small Molecule Biochemistry	1.00E-03
3	Cellular Development, Cellular Growth and Proliferation, Respiratory System Development and Function	1.00E-14	3	Cellular Development, Cellular Growth and Proliferation, Endocrine System Development and Function	1.00E-21
4	Cancer, Carbohydrate Metabolism, Endocrine System Disorders	1.00E-03	4	Cellular Movement, DNA Replication, Recombination, and Repair, Gene Expression	1.00E-11
5	Cell Death, Post-Translational Modification, Amino Acid Metabolism	1.00E-03	5	Cellular Compromise, Cell Morphology, Inflammatory Response	1.00E-02

The genome-wide gene expression profile was studied on Human Gene 1.0 ST array in U373MG cells following a 24 hour-exposure to the vehicle (DMSO) or to the three chemical compounds (Figure 1). By importing the complete list of Entrez Gene IDs of downregulated or upregulated genes into IPA, the molecular networks related to the set of imported genes were identified. The networks are listed with the functional category and p-value.