FIG. 6.
Interactions between partners of the SCL complex. Pulldown experiments were performed with immobilized GST, GST-GATA-1, GST-LMO2, GST-SCL, and GST-Sp1 as well as with 35S-labeled SCL, Ldb1, LMO2, GATA-1, and luciferase. Protein signals were quantified using ImageQuant software, and binding efficiency levels (percentages of input) were calculated in comparison to those of input samples (10%) after subtraction of background GST signals. The lower panel shows bacterially expressed GST fusion proteins, which were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and stained with Coomassie blue.