Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Pol II-Med does not function in transcription reinitiation. Scaffold complexes were generated by ATP treatment of PICs formed on immobilized promoter templates. Immobilized scaffolds were complemented with TFIIB and the indicated amounts of Pol II and TFIIF, Pol II-Med and TFIIF, or Pol II-TFIIF. Single- and multiround transcription assays were as described in Materials and Methods. Shown are results of RNA analysis by primer extension and quantitation of primer extension products.
