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. Author manuscript; available in PMC: 2012 Sep 14.
Published in final edited form as: Carbohydr Res. 2011 May 12;346(12):1576–1580. doi: 10.1016/j.carres.2011.05.005

Figure 2.

Figure 2

Glycosylation reactions were performed in solution using an azido modified substrate in order to determine the kinetic parameters for UDP-GlcNAc and the synthesized analog. Reactions were terminated at different time points and then applied to the monolayers to allow immobilization and SAMDI characterization of both the lactose substrate and trisaccharide product. The lower panel shows the spectrum from a 120 min reaction of UDP(βS)-GlcNAc. Black and red label the peaks of lactose and the trisaccharide products, respectively. Letters in parenthesis showed the different ion adducts appearing in the spectrum.